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hydrocortisone  (PromoCell)


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    PromoCell hydrocortisone
    Hydrocortisone, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/endothelial+cell+growth+medium+mv+2/Endothelial+Cell+Growth+Medium+MV+2+KIT/pmc13019081-439-15-16
    Average 96 stars, based on 121 article reviews
    hydrocortisone - by Bioz Stars, 2026-09
    96/100 stars

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    Cell Culture:

    Article Title: Cell culture methods and compositions
    Article Snippet: Cells are subsequently passaged using TrypLE Express (Life Technologies) and seeded onto respective conditions: no substrate, Geltrex (Life Technologies), NID1 (Nidogen-1) (Merck), COL1A1 (Collagen I) (Southern Biotech), COL4A1 (Collagen 4) (Merck), COL6A1 (Collagen 6) (Rockland), THBS1 (Thrombospondin 1) (Merck), FGF10 (Fibroblast Growth Factor-10) (Stemcell Technologies), FGF7 (Fibroblast Growth Factor-7) (Stemcell Technologies) and all ligands (a combination of aforementioned substrates and ligands without Geltrex). .. Endothelial cells are cultured in Endothelial Cell Growth Medium MV 2 (PromoCell, C-22022) supplemented with supplement mix containing fetal calf serum (0.05 ml/ml), epidermal growth factor (recombinant human) (5 ng/ml), basic fibroblast growth factor (recombinant human) (10 ng/ml), insulin-like growth factor (Long R3 IGF) (20 ng/ml), vascular endothelial growth factor (recombinant human) (0.5 ng/ml), ascorbic acid (1 μg/ml) and hydrocortisone (0.2 μg/ml). .. Cells are subsequently passaged using TrypLE Express (Life Technologies) and seeded onto respective conditions: no substrate, Geltrex (Life Technologies), FN1 (Fibronectin) (Merck), THBS1 (Thrombospondin 1) (Merck), CTGF (Connective tissue growth factor) (Life Technologies), PDGFB (Platelet-Derived Growth Factor Subunit B) (Stemcell Technologies), CYR61 (Cysteine-rich angiogenic inducer 61) (Novus), BMP4 (Bone Morphogenetic Protein 4) (Life Technologies), BMP6 (Bone Morphogenetic Protein 6) (Merck) and all ligands (a combination of aforementioned substrates and ligands without Geltrex).

    Article Title: Targeting C2 reduces ischemia-reperfusion injury-induced complement activation in preclinical human models
    Article Snippet: HUVEC cells (CRL-1730TM, ATCC®) were cultured in endothelial cell growth basal medium-plus (Lonza) and EBM-Plus SingleQuots additives (Lonza), supplemented with 0.1 μg Primocin (Bio-Connect) at 5% CO 2 and 37 °C. .. Primary lung ECs and primary kidney ECs were collected from lung or kidney graft perfusates, respectively, and cultured as previously described using Endothelial Cell Growth Medium MV 2 (Promocell) . ..

    Article Title: Lymphatic vessel dysfunction contributes to severe dengue pathogenesis
    Article Snippet: Primary Human Dermal Lymphatic Endothelial Cells (HDLEC, C-12216) from juvenile foreskin and human umbilical vein endothelial cells (HUVECs, C-12203) from pooled donors were obtained from Promocell (Heidelberg, Germany). .. HDLECs were cultured Endothelial Cell Growth Medium MV 2 (EGM-MV2, #C-22022) and HUVECs in Endothelial Growth Medium 2 (EGM-2) (Promocell) in an incubator set at 370C with 5% CO2. .. Upon reaching 80% confluency, cells were subjected to trypsinization using DetachKit (PromoCell), according to the manufacturer’s instructions.

    Article Title: Generation of hiPSC-Derived Brain Microvascular Endothelial Cells Using Directed Differentiation and Transcriptional Reprogramming
    Article Snippet: Cell suspensions were washed with FACS buffer, and cell pellets were resuspended for FACS by filtering through 0.45-μM filters (Fisher Scientific) to remove cell aggregates. bpECs or rBMECs were sorted on the Sony MA900 cell sorter, and CD31+/VE-cadherin+ cells were subsequently expanded. .. HBMECs (Cell Systems) were cultured in Endothelial Cell Growth Medium MV 2 (Promocell). .. Pericytes were cultured in pericyte media (PM; ScienCell), and astrocytes were cultured in astrocyte media (AM; ScienCell).

    Article Title: Generation of hiPSC-Derived Brain Microvascular Endothelial Cells Using Directed Differentiation and Transcriptional Reprogramming
    Article Snippet: Cell suspensions were washed with FACS buffer, and cell pellets were resuspended for FACS by filtering through 0.45-μM filters (Fisher Scientific) to remove cell aggregates. bpECs or rBMECs were sorted on the Sony MA900 cell sorter, and CD31 + /VE-cadherin + cells were subsequently expanded. .. HBMECs (Cell Systems) were cultured in Endothelial Cell Growth Medium MV 2 (Promocell). .. Pericytes were cultured in pericyte media (PM; ScienCell), and astrocytes were cultured in astrocyte media (AM; ScienCell).

    Recombinant:

    Article Title: Cell culture methods and compositions
    Article Snippet: Cells are subsequently passaged using TrypLE Express (Life Technologies) and seeded onto respective conditions: no substrate, Geltrex (Life Technologies), NID1 (Nidogen-1) (Merck), COL1A1 (Collagen I) (Southern Biotech), COL4A1 (Collagen 4) (Merck), COL6A1 (Collagen 6) (Rockland), THBS1 (Thrombospondin 1) (Merck), FGF10 (Fibroblast Growth Factor-10) (Stemcell Technologies), FGF7 (Fibroblast Growth Factor-7) (Stemcell Technologies) and all ligands (a combination of aforementioned substrates and ligands without Geltrex). .. Endothelial cells are cultured in Endothelial Cell Growth Medium MV 2 (PromoCell, C-22022) supplemented with supplement mix containing fetal calf serum (0.05 ml/ml), epidermal growth factor (recombinant human) (5 ng/ml), basic fibroblast growth factor (recombinant human) (10 ng/ml), insulin-like growth factor (Long R3 IGF) (20 ng/ml), vascular endothelial growth factor (recombinant human) (0.5 ng/ml), ascorbic acid (1 μg/ml) and hydrocortisone (0.2 μg/ml). .. Cells are subsequently passaged using TrypLE Express (Life Technologies) and seeded onto respective conditions: no substrate, Geltrex (Life Technologies), FN1 (Fibronectin) (Merck), THBS1 (Thrombospondin 1) (Merck), CTGF (Connective tissue growth factor) (Life Technologies), PDGFB (Platelet-Derived Growth Factor Subunit B) (Stemcell Technologies), CYR61 (Cysteine-rich angiogenic inducer 61) (Novus), BMP4 (Bone Morphogenetic Protein 4) (Life Technologies), BMP6 (Bone Morphogenetic Protein 6) (Merck) and all ligands (a combination of aforementioned substrates and ligands without Geltrex).



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    Brain pericyte secretions mediate the inhibition of TNBC cell adhesion to the BBB. (A) Experimental workflow for adhesion assay. All BBB experiments were performed using <t>ECGMV2</t> medium with supplements for all conditions from day −6 to the end of the experiment. The astrocyte medium was renewed 24 h before the adhesion experiment with the same ECGMV2 medium. Endothelial cells (ECs) and brain pericytes (hBPs) were co‐cultured for 5 days, and inserts containing brain‐like endothelial cells (hBLECs) were then either maintained with hBPs or transferred to wells without hBPs. After 24 hours, and prior to MDA‐MB‐231 cells seeding, inserts were subjected to the following conditions: maintained without hBPs (hBLEC 24h); transferred to hBPs previously cultured alone for 24 hours (hBLEC 24h then hBP); transferred to wells without hBPs but containing either culture medium (hBLEC) or CM from co‐culture (hBLEC + CM coc ); transferred to wells with astrocytes (hBLEC + A) or maintained in co‐culture with hBPs (hBLEC + hBP). (B–G), Quantification (B, D, F) of MDA‐MB‐231 cell adhesion to hBLECs after 3 h of incubation and representative images (C, E, G) of adhered MDA‐MB‐231 cells (green) under the different conditions. Data are obtained from three independent experiments, with three technical replicates per condition. Statistical analyses were performed using one‐way ANOVA followed by Tukey's test (B), Welch's ANOVA followed by Dunnett's T3 test (D) and Kruskal–Wallis test followed by Dunn's test (F). CM coc = conditioned medium from 24 h hBLECs and hBPs coculture, A = astrocytes, hBP mono = 24 h hBPs monoculture. Scale bar = 750 µm. BBB, blood–brain barrier; ECGMV2, EC Growth Medium MV 2; ECs, endothelial cells; hBLECs, human brain‐like endothelial cells; ns, non‐significant; hBPs, human brain pericytes; TNBC, triple‐negative breast cancer. **** p ≤ 0.0001; * p ≤ 0.05.
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    Brain pericyte secretions mediate the inhibition of TNBC cell adhesion to the BBB. (A) Experimental workflow for adhesion assay. All BBB experiments were performed using <t>ECGMV2</t> medium with supplements for all conditions from day −6 to the end of the experiment. The astrocyte medium was renewed 24 h before the adhesion experiment with the same ECGMV2 medium. Endothelial cells (ECs) and brain pericytes (hBPs) were co‐cultured for 5 days, and inserts containing brain‐like endothelial cells (hBLECs) were then either maintained with hBPs or transferred to wells without hBPs. After 24 hours, and prior to MDA‐MB‐231 cells seeding, inserts were subjected to the following conditions: maintained without hBPs (hBLEC 24h); transferred to hBPs previously cultured alone for 24 hours (hBLEC 24h then hBP); transferred to wells without hBPs but containing either culture medium (hBLEC) or CM from co‐culture (hBLEC + CM coc ); transferred to wells with astrocytes (hBLEC + A) or maintained in co‐culture with hBPs (hBLEC + hBP). (B–G), Quantification (B, D, F) of MDA‐MB‐231 cell adhesion to hBLECs after 3 h of incubation and representative images (C, E, G) of adhered MDA‐MB‐231 cells (green) under the different conditions. Data are obtained from three independent experiments, with three technical replicates per condition. Statistical analyses were performed using one‐way ANOVA followed by Tukey's test (B), Welch's ANOVA followed by Dunnett's T3 test (D) and Kruskal–Wallis test followed by Dunn's test (F). CM coc = conditioned medium from 24 h hBLECs and hBPs coculture, A = astrocytes, hBP mono = 24 h hBPs monoculture. Scale bar = 750 µm. BBB, blood–brain barrier; ECGMV2, EC Growth Medium MV 2; ECs, endothelial cells; hBLECs, human brain‐like endothelial cells; ns, non‐significant; hBPs, human brain pericytes; TNBC, triple‐negative breast cancer. **** p ≤ 0.0001; * p ≤ 0.05.
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    Brain pericyte secretions mediate the inhibition of TNBC cell adhesion to the BBB. (A) Experimental workflow for adhesion assay. All BBB experiments were performed using <t>ECGMV2</t> medium with supplements for all conditions from day −6 to the end of the experiment. The astrocyte medium was renewed 24 h before the adhesion experiment with the same ECGMV2 medium. Endothelial cells (ECs) and brain pericytes (hBPs) were co‐cultured for 5 days, and inserts containing brain‐like endothelial cells (hBLECs) were then either maintained with hBPs or transferred to wells without hBPs. After 24 hours, and prior to MDA‐MB‐231 cells seeding, inserts were subjected to the following conditions: maintained without hBPs (hBLEC 24h); transferred to hBPs previously cultured alone for 24 hours (hBLEC 24h then hBP); transferred to wells without hBPs but containing either culture medium (hBLEC) or CM from co‐culture (hBLEC + CM coc ); transferred to wells with astrocytes (hBLEC + A) or maintained in co‐culture with hBPs (hBLEC + hBP). (B–G), Quantification (B, D, F) of MDA‐MB‐231 cell adhesion to hBLECs after 3 h of incubation and representative images (C, E, G) of adhered MDA‐MB‐231 cells (green) under the different conditions. Data are obtained from three independent experiments, with three technical replicates per condition. Statistical analyses were performed using one‐way ANOVA followed by Tukey's test (B), Welch's ANOVA followed by Dunnett's T3 test (D) and Kruskal–Wallis test followed by Dunn's test (F). CM coc = conditioned medium from 24 h hBLECs and hBPs coculture, A = astrocytes, hBP mono = 24 h hBPs monoculture. Scale bar = 750 µm. BBB, blood–brain barrier; ECGMV2, EC Growth Medium MV 2; ECs, endothelial cells; hBLECs, human brain‐like endothelial cells; ns, non‐significant; hBPs, human brain pericytes; TNBC, triple‐negative breast cancer. **** p ≤ 0.0001; * p ≤ 0.05.
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    Brain pericyte secretions mediate the inhibition of TNBC cell adhesion to the BBB. (A) Experimental workflow for adhesion assay. All BBB experiments were performed using <t>ECGMV2</t> medium with supplements for all conditions from day −6 to the end of the experiment. The astrocyte medium was renewed 24 h before the adhesion experiment with the same ECGMV2 medium. Endothelial cells (ECs) and brain pericytes (hBPs) were co‐cultured for 5 days, and inserts containing brain‐like endothelial cells (hBLECs) were then either maintained with hBPs or transferred to wells without hBPs. After 24 hours, and prior to MDA‐MB‐231 cells seeding, inserts were subjected to the following conditions: maintained without hBPs (hBLEC 24h); transferred to hBPs previously cultured alone for 24 hours (hBLEC 24h then hBP); transferred to wells without hBPs but containing either culture medium (hBLEC) or CM from co‐culture (hBLEC + CM coc ); transferred to wells with astrocytes (hBLEC + A) or maintained in co‐culture with hBPs (hBLEC + hBP). (B–G), Quantification (B, D, F) of MDA‐MB‐231 cell adhesion to hBLECs after 3 h of incubation and representative images (C, E, G) of adhered MDA‐MB‐231 cells (green) under the different conditions. Data are obtained from three independent experiments, with three technical replicates per condition. Statistical analyses were performed using one‐way ANOVA followed by Tukey's test (B), Welch's ANOVA followed by Dunnett's T3 test (D) and Kruskal–Wallis test followed by Dunn's test (F). CM coc = conditioned medium from 24 h hBLECs and hBPs coculture, A = astrocytes, hBP mono = 24 h hBPs monoculture. Scale bar = 750 µm. BBB, blood–brain barrier; ECGMV2, EC Growth Medium MV 2; ECs, endothelial cells; hBLECs, human brain‐like endothelial cells; ns, non‐significant; hBPs, human brain pericytes; TNBC, triple‐negative breast cancer. **** p ≤ 0.0001; * p ≤ 0.05.
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    Brain pericyte secretions mediate the inhibition of TNBC cell adhesion to the BBB. (A) Experimental workflow for adhesion assay. All BBB experiments were performed using ECGMV2 medium with supplements for all conditions from day −6 to the end of the experiment. The astrocyte medium was renewed 24 h before the adhesion experiment with the same ECGMV2 medium. Endothelial cells (ECs) and brain pericytes (hBPs) were co‐cultured for 5 days, and inserts containing brain‐like endothelial cells (hBLECs) were then either maintained with hBPs or transferred to wells without hBPs. After 24 hours, and prior to MDA‐MB‐231 cells seeding, inserts were subjected to the following conditions: maintained without hBPs (hBLEC 24h); transferred to hBPs previously cultured alone for 24 hours (hBLEC 24h then hBP); transferred to wells without hBPs but containing either culture medium (hBLEC) or CM from co‐culture (hBLEC + CM coc ); transferred to wells with astrocytes (hBLEC + A) or maintained in co‐culture with hBPs (hBLEC + hBP). (B–G), Quantification (B, D, F) of MDA‐MB‐231 cell adhesion to hBLECs after 3 h of incubation and representative images (C, E, G) of adhered MDA‐MB‐231 cells (green) under the different conditions. Data are obtained from three independent experiments, with three technical replicates per condition. Statistical analyses were performed using one‐way ANOVA followed by Tukey's test (B), Welch's ANOVA followed by Dunnett's T3 test (D) and Kruskal–Wallis test followed by Dunn's test (F). CM coc = conditioned medium from 24 h hBLECs and hBPs coculture, A = astrocytes, hBP mono = 24 h hBPs monoculture. Scale bar = 750 µm. BBB, blood–brain barrier; ECGMV2, EC Growth Medium MV 2; ECs, endothelial cells; hBLECs, human brain‐like endothelial cells; ns, non‐significant; hBPs, human brain pericytes; TNBC, triple‐negative breast cancer. **** p ≤ 0.0001; * p ≤ 0.05.

    Journal: Journal of Cell Communication and Signaling

    Article Title: Human brain pericytes protect the blood–brain barrier from triple‐negative breast cancer cells while promoting tumor aggressiveness

    doi: 10.1002/ccs3.70070

    Figure Lengend Snippet: Brain pericyte secretions mediate the inhibition of TNBC cell adhesion to the BBB. (A) Experimental workflow for adhesion assay. All BBB experiments were performed using ECGMV2 medium with supplements for all conditions from day −6 to the end of the experiment. The astrocyte medium was renewed 24 h before the adhesion experiment with the same ECGMV2 medium. Endothelial cells (ECs) and brain pericytes (hBPs) were co‐cultured for 5 days, and inserts containing brain‐like endothelial cells (hBLECs) were then either maintained with hBPs or transferred to wells without hBPs. After 24 hours, and prior to MDA‐MB‐231 cells seeding, inserts were subjected to the following conditions: maintained without hBPs (hBLEC 24h); transferred to hBPs previously cultured alone for 24 hours (hBLEC 24h then hBP); transferred to wells without hBPs but containing either culture medium (hBLEC) or CM from co‐culture (hBLEC + CM coc ); transferred to wells with astrocytes (hBLEC + A) or maintained in co‐culture with hBPs (hBLEC + hBP). (B–G), Quantification (B, D, F) of MDA‐MB‐231 cell adhesion to hBLECs after 3 h of incubation and representative images (C, E, G) of adhered MDA‐MB‐231 cells (green) under the different conditions. Data are obtained from three independent experiments, with three technical replicates per condition. Statistical analyses were performed using one‐way ANOVA followed by Tukey's test (B), Welch's ANOVA followed by Dunnett's T3 test (D) and Kruskal–Wallis test followed by Dunn's test (F). CM coc = conditioned medium from 24 h hBLECs and hBPs coculture, A = astrocytes, hBP mono = 24 h hBPs monoculture. Scale bar = 750 µm. BBB, blood–brain barrier; ECGMV2, EC Growth Medium MV 2; ECs, endothelial cells; hBLECs, human brain‐like endothelial cells; ns, non‐significant; hBPs, human brain pericytes; TNBC, triple‐negative breast cancer. **** p ≤ 0.0001; * p ≤ 0.05.

    Article Snippet: Differentiated ECs were cultured in 100‐mm Petri dishes (Corning) coated with gelatin (Sigma‐Aldrich), in EC growth medium MV 2 (ECGMV2 + Supplement mix C‐39226, PromoCell) and gentamicin (50 μg/ml; Biowest). hBPs (female) were provided by Dr. Fumitaka Shimizu and Pr.

    Techniques: Inhibition, Cell Adhesion Assay, Cell Culture, Co-Culture Assay, Incubation

    Brain pericytes limit TNBC cell adhesion to the BBB under hypoxic conditions. (A) Experimental workflow for adhesion assay in hypoxic conditions. All BBB experiments were performed using ECGMV2 medium with supplements for all conditions from day −6 to the end of the experiment. After 5 days of co‐culture with brain pericytes (hBPs), inserts containing brain‐like endothelial cells (hBLECs) were exposed to hypoxia for 24 h in the presence or absence of hBPs. Parallel conditions maintained in normoxia served as controls. (B–E) Quantification (B, D) of MDA‐MB‐231 cell adhesion to hBLECs after 3 h of incubation and representative images (C, E) of adhered MDA‐MB‐231 cells (green) under the different conditions. Data are obtained from three independent experiments, with three technical replicates per condition. Statistical analyses were performed using Welch's ANOVA followed by Dunnett's T3 test (B) and Kruskal‐Wallis test followed by Dunn's test (D). hBP‐CM mono = conditioned medium from 24 h hBPs monoculture. Scale bar = 750 μm. BBB, blood–brain barrier; ECGMV2, EC Growth Medium MV 2; hBLECs, human brain‐like endothelial cells; hBPs, human brain pericytes; ns, non‐significant; TNBC, triple‐negative breast cancer. *** p ≤ 0.001; ** p ≤ 0.01; * p ≤ 0.05.

    Journal: Journal of Cell Communication and Signaling

    Article Title: Human brain pericytes protect the blood–brain barrier from triple‐negative breast cancer cells while promoting tumor aggressiveness

    doi: 10.1002/ccs3.70070

    Figure Lengend Snippet: Brain pericytes limit TNBC cell adhesion to the BBB under hypoxic conditions. (A) Experimental workflow for adhesion assay in hypoxic conditions. All BBB experiments were performed using ECGMV2 medium with supplements for all conditions from day −6 to the end of the experiment. After 5 days of co‐culture with brain pericytes (hBPs), inserts containing brain‐like endothelial cells (hBLECs) were exposed to hypoxia for 24 h in the presence or absence of hBPs. Parallel conditions maintained in normoxia served as controls. (B–E) Quantification (B, D) of MDA‐MB‐231 cell adhesion to hBLECs after 3 h of incubation and representative images (C, E) of adhered MDA‐MB‐231 cells (green) under the different conditions. Data are obtained from three independent experiments, with three technical replicates per condition. Statistical analyses were performed using Welch's ANOVA followed by Dunnett's T3 test (B) and Kruskal‐Wallis test followed by Dunn's test (D). hBP‐CM mono = conditioned medium from 24 h hBPs monoculture. Scale bar = 750 μm. BBB, blood–brain barrier; ECGMV2, EC Growth Medium MV 2; hBLECs, human brain‐like endothelial cells; hBPs, human brain pericytes; ns, non‐significant; TNBC, triple‐negative breast cancer. *** p ≤ 0.001; ** p ≤ 0.01; * p ≤ 0.05.

    Article Snippet: Differentiated ECs were cultured in 100‐mm Petri dishes (Corning) coated with gelatin (Sigma‐Aldrich), in EC growth medium MV 2 (ECGMV2 + Supplement mix C‐39226, PromoCell) and gentamicin (50 μg/ml; Biowest). hBPs (female) were provided by Dr. Fumitaka Shimizu and Pr.

    Techniques: Cell Adhesion Assay, Co-Culture Assay, Incubation

    Brain pericytes maintain BBB integrity in the presence of TNBC cells under hypoxic conditions and supplement deprivation. (A) After 5 days of co‐culture with brain pericytes (hBPs) in ECGMV2 medium with supplements, inserts containing brain‐like endothelial cells (hBLECs) co‐cultured with hBPs were exposed to hypoxia for 16 h following medium replacement with ECGMV2 basal medium (without supplements). Then, endothelial permeability (Pe) to Lucifer Yellow was assessed after 3 h of incubation with MDA‐MB‐231 cells, in the presence or absence of hBPs, under normoxic and hypoxic conditions. (B) Permeability values (expressed in x10 −3 cm/min ± SD) are summarized in a table. (C) Representative images of endothelial Claudin‐5 (magenta) immunostaining in the absence or presence of hBPs after 3 h of incubation with MDA‐MB‐231 cells (green) under hypoxic conditions in basal medium. Data are obtained from three independent experiments, with three technical replicates per condition. Statistical analyses were performed using Welch's ANOVA followed by Dunnett's T3 test. MDA = MDA‐MB‐231 cells. Scale bars = 300 μm (10X) and 100 μm (40X). BBB, blood–brain barrier; ECGMV2, EC Growth Medium MV 2; hBLECs, human brain‐like endothelial cells; hBPs, human brain pericytes; ns, non‐significant; TNBC, triple‐negative breast cancer. *** p ≤ 0.001; ** p ≤ 0.01; * p ≤ 0.05.

    Journal: Journal of Cell Communication and Signaling

    Article Title: Human brain pericytes protect the blood–brain barrier from triple‐negative breast cancer cells while promoting tumor aggressiveness

    doi: 10.1002/ccs3.70070

    Figure Lengend Snippet: Brain pericytes maintain BBB integrity in the presence of TNBC cells under hypoxic conditions and supplement deprivation. (A) After 5 days of co‐culture with brain pericytes (hBPs) in ECGMV2 medium with supplements, inserts containing brain‐like endothelial cells (hBLECs) co‐cultured with hBPs were exposed to hypoxia for 16 h following medium replacement with ECGMV2 basal medium (without supplements). Then, endothelial permeability (Pe) to Lucifer Yellow was assessed after 3 h of incubation with MDA‐MB‐231 cells, in the presence or absence of hBPs, under normoxic and hypoxic conditions. (B) Permeability values (expressed in x10 −3 cm/min ± SD) are summarized in a table. (C) Representative images of endothelial Claudin‐5 (magenta) immunostaining in the absence or presence of hBPs after 3 h of incubation with MDA‐MB‐231 cells (green) under hypoxic conditions in basal medium. Data are obtained from three independent experiments, with three technical replicates per condition. Statistical analyses were performed using Welch's ANOVA followed by Dunnett's T3 test. MDA = MDA‐MB‐231 cells. Scale bars = 300 μm (10X) and 100 μm (40X). BBB, blood–brain barrier; ECGMV2, EC Growth Medium MV 2; hBLECs, human brain‐like endothelial cells; hBPs, human brain pericytes; ns, non‐significant; TNBC, triple‐negative breast cancer. *** p ≤ 0.001; ** p ≤ 0.01; * p ≤ 0.05.

    Article Snippet: Differentiated ECs were cultured in 100‐mm Petri dishes (Corning) coated with gelatin (Sigma‐Aldrich), in EC growth medium MV 2 (ECGMV2 + Supplement mix C‐39226, PromoCell) and gentamicin (50 μg/ml; Biowest). hBPs (female) were provided by Dr. Fumitaka Shimizu and Pr.

    Techniques: Co-Culture Assay, Cell Culture, Permeability, Incubation, Immunostaining